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张学斌
发布时间:2019年01月02日 16:31    作者:



张学斌

职称:教授(特聘)

联系电话:86-13467258356

E-mailxuebinzhang@henu.edu.cn; 或者xuebin.zhang2000@gmail.com

研究方向:植物次生代谢调控、植物F-box基因家族功能解析、植物代谢组学



张学斌,男,博士,教授,博士生导师。

    1998至 2005年就读于山西农业大学食品科学与工程学院及文理学院。2005年起就读于英国诺丁汉大学植物科学系,2008年获英国诺丁汉大学理学博士学位。2008至 2011年在英国洛桑实验站(Rothamsted Research)从事博士后工作。2011年3月至2018年4月就职于美国国家能源部布鲁克海文国家实验室(Brookhaven National Laboratory)。主要从事植物苯丙素类化合物合成途径调控和F-box基因家族功能解析方面的研究。分离并鉴定了多个直接控制苯丙素合成途径关键结构酶蛋白降解的F-BOX 基因;该类研究成果引领了植物苯丙素类化合物合成过程中蛋白降解调控机制的研究。在Nature Communications, Plant Biotechnology, The Plant Cell,Plant Physiology,Molecular plant, Biochemical Journal,FEBS letter等国际知名学术期刊发表研究论文18篇。申请发明专利1项。


主要研究方向:

1. 系统研究苯丙素代谢途径的翻译后调控新机制:

    高等植物通过莽草酸途径合成的苯丙氨酸、酪氨酸是苯丙烷化合物合成的起始分子。苯丙氨酸在苯丙氨酸解氨酶(phenylalanine ammonia lyase,PAL)的催化下形成肉桂酸,再经由一系列的羟基化、甲基化等修饰进而转化为木质素单体的一系列酶促反应过程是苯丙烷类化合物代谢的中心途径。以往对苯丙烷代谢途径调控的研究都主要集中在转录水平。苯丙素代谢途径响应各种生物或者非生物逆境诱导如高糖、高盐、低温、机械损伤、病原菌感染、光、毒素处理、昆虫取食等。课题组已发表工作说明翻译后调控是植物保留的一种新的、更有效的苯丙素代谢的调控机制。本课题组将基于前期工作基础继续探讨和系统化研究苯丙素代谢途径中除PAL和CHS以外其他关键结构酶的翻译后调控分子机理。

2. 蛋白互作组学方法解析植物F-box家族基因功能(F-box protein interactome):

    F-box蛋白是典型的E3复合体SCF的组成部分,SCF介导靶蛋白范素化-降解机制是细胞内大部分蛋白的通用降解途径。F-box基因家族存在于各类动、植物中,并且家族成员数目庞大。在模型植物拟南芥中就有超过700个F-box基因,其中KFB(Kelch containing F-box protein)蛋白作为F-box 蛋白家族的1个亚类也有近100个成员之多。现有为数不多的研究表明F-box蛋白在植物生长发育、代谢调控、逆境适应、信号转导过程中所发挥的重要作用。F-box 蛋白的靶蛋白身份确定以后,其生物功能的解析也就迎刃而解。但由于常规的蛋白互作手段很难作为筛选工具来捕捉F-box蛋白和其靶蛋白的相互作用,已鉴定出的靶蛋白屈指可数,因此人们对F-box基因家族成员生物功能的了解还非常有限。课题组将在已有的工作基础上继续系统发掘植物F-box基因家族功能;建立高可信度的F-box家族蛋白和其靶蛋白的互作网络图谱;以经济农作物棉花为材料,运用所获理论知识探讨关键农艺形状与同源F-box基因的生物功能的相关性。相关研究将有助于我们更加深入的了解F-box蛋白在植物重要生理性状形成过程中所发挥的重要作用。随着研究的深入, 人们对这一领域将会有更清晰的认识,研究成果也对F-box功能基因组学的研究有重要意义。

3. 苯丙素代谢在棉花抵御病、虫害过程中的作用机理以及对棉纤维品质影响的分子基础:

    苯丙烷类和类黄酮类天然产物在棉花抗生物逆境过程中发挥着重要的作用。在黄萎病发病过程中苯丙氨酸脱氨酶(PAL) 和过氧化物酶(POD)活性大副升高。通过基因工程手段过量表达漆酶(LAC)使棉花叶片、茎杆细胞适量木质化可以有效提高棉花对黄萎病、棉铃虫、棉蚜的抵御能力。适量木质素合成有利于棉花纤维细胞的伸长,相反类黄酮含量过高(如:彩棉)会导致纤维品质的下降,但是其中的详细分子机理和相关性还不清楚,相关研究也成为近年棉花育种的热点方向。随着测序技术的不断完善,棉花研究领域积累了大量的基因组、转录组、表观组数据。但棉花代谢组学研究才刚刚开始。基于课题组多年的次生代谢调控的理论研究的基础上,拟利用现代比较基因组学结合代谢组学方法探讨苯丙烷和类黄酮代谢在棉花抵御病、虫害过程及棉纤维品质形成过程中的分子基础。


主持的重要研究项目:

河南大学人才特区支持计划。


代表性研究论文和著作(*为通讯作者):

1. Zhang X*, Gonzalez-Carranza H. Z, Zhang S, Miao Y, Liu C, Roberts J. (2019, in press). F-box proteins in plants. Annual Plant Review online, February, 2019

2. Zhang, X, Zhang, J, Li, W, Yang, Y, Qin, P, Zhang, X, Lu, M. (2018) Magnetic graphene oxide nanocomposites as the adsorbent for extraction and pre-concentration of azo dyes in different food samples followed by high-performance liquid chromatography analysis.  Food Additives & Contaminants: Part A. 2018

3. Zhang, X., Jayaweera D, Peters J L, Szecsi J, Bendahmane M, Roberts J A, González-Carranza Z H*. (2017). The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway. PLoS ONE , 2017, IF: 2.806

4. Zhang, X., Abrahanb, C., Colquhounb, T.A and Liu, C. (2017). A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis. The Plant Cell, May, 2017. IF 10.125. (*Editor’s choice of cover story of the month)

5. Zhang, X., Gou, M., Guo, C., Yang H., and Liu, C. (2015). Down-regulation of the kelch domaincontaining F-box protein in Arabidopsis enhances the production of (poly)phenols and tolerance to UV-radiation. Plant Physiology, January, 2015. IF: 7.908.

6. Zhang, X., and Liu, C. (2014). Multifaceted regulations of gateway enzymes in the biosynthesis of phenylpropanoids. Molecular Plant, November, 2014. IF: 6.126.

7. Zhang, X., Gou, M., Liu, C. (2013). Kelch Repeat F-box Proteins Regulate Phenylpropanoid Biosynthesis via Controlling the Turnover of Phenylalanine Ammonia-Lyase. The Plant Cell, 25, 4994-5010. IF 10.125.

8. Zhang, X., De Marcos Lousa, C., Shutte-Lensink, N., Ofman, R., Wanders, R.J., Baldwin, S.A., Baker, A., Kemp, S., and Theodoulou, F.L. (2011), Conservation of targeting but divergence in function and quality control of peroxisomal ABC transporters: an analysis using crosskingdom expression. Biochemical Journal, 436, 547-57. IF: 5.0.

9. Nyathi, Y*., Zhang, X*., Baldwin, J.M., Bernhardt, K., Johnson, B., Baldwin, S.A., Theodoulou, F.L., and Baker, A. (2012). Pseudo half-molecules of the ABC transporter, COMATOSE, bind Pex19 and target to peroxisomes independently but both are required for activity. FEBS Letters. 586, 2280-2286. (*co-first author). IF: 4.0.

10. Cheng A, Zhang X, Han X-J, Zhang Y-Y, Gao S, Liu C-J, Lou H. (2017) Identification of Chalcone Isomerase in the Basal Land Plants Reveals an Ancient Evolution of Enzymatic Cyclization Activity for Synthesis of Flavonoids. New Phytologist , September, 2017, IF: 7.330

11. Gonzalez-Carranza H Z *, Zhang X Peters L JVeronique Szecsi B, Bendahmane J, M Roberts A J. (2017) Hawaiian skirt controls size and floral organ number by modulating CUC1 and CUC2 expression. PLoS ONE ,September 20, 2017, IF: 2.806

12. Gou, M., Hou, G., Yang, H., Zhang, X, Cai, Y., Kai, G and Liu, C. (2016). The MYB107 transcription factor positively regulates suberin biosynthesis. Plant Physiology, December, 2016. IF: 7.908.

13. Cai, Y., Zhang, K., Kim, H., Hou, G., Zhang, X., Yang, H., Feng, H., Miller, L., Ralph, J & Liu, C. (2016). Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase. Nature Communications, Article number 11989. IF: 10.2.

14. Liu, C., Cai, Y., Zhang, X., Gou, M., and Yang, H. (2014). Tailoring lignin biosynthesis for efficient and sustainable biofuel production. Plant Biotechnology Journal, September, 2014. IF: 6.279.

15. Mendiondo, G.M., Medhurst, A., Roermund, C.W., Zhang, X., Devonshire, J., Scholefield, D., Fernández, J., Axcell, B., Ramsay, L., Waterham, H.R., Waugh, R., Theodoulou, F.L. and Holdsworth, M.J. (2014). Barley has two peroxisomal ABC transporters with multiple functions in β-oxidation. Journal of Experimental Botany, June 9, 2014. IF: 5.542.

16. Zhang, K., Novak, O., Wei, Z., Gou, M., Zhang, X., Yu, Y., Yang, H., Cai, Y., Strnad, M., and Liu, C. (2014). Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins. Nature Communications, Article number 3274. IF: 10.2.

17. Fan, J., Yan, C., Zhang, X., and Xu, C. (2013). Dual Role for Phospholipid:Diacylglycerol Acyltransferase: Enhancing Fatty Acid Synthesis and Diverting Fatty Acids from Membrane Lipids to Triacylglycerol in Arabidopsis Leaves. The Plant Cell, 25, 3506-18.IF: 10.125.

18. Theodoulou, F.L., Nyathi, Y., van Roermund, C., Lousa, C.D., Linka, N., Zhang, X., Haslam, R., Napier, J.A., Baldwin, S.A., Weber, A., and Baker, A. (2010). Peroxisomal transporters associated with beta-oxidation. Biochimica et Biophysica Acta-Bioenergetics 1797,145154, IF: 5.0.

Book Chapter:

Theodoulou, F.L., Zhang, X., De Marcos Lousa, C., Nyathi, Y., and Baker, A. (2011). Peroxisomal transport systems: roles in signalling and metabolism. In: Transporters and pumps in plant signalling. Eds. Markus Geisler and Kees Venema. Signaling and Communication in Plants 7, Springer-Verlag Berlin Heidelberg. pp 327-351


专利

“Enhancing Oil Accumulation in Vegetative Tissue of Plants” Publication number: US 2014/0031573 A1 (US department of Energy); Publication date: Jan. 30, 2014.


重要奖项:

Spot Light Award in BNL, USA (2014)

Top-10 Brookhaven National Laboratory Breakthroughs (2013)

Company of Biologists. Travel award to attend the SEB annual meeting in Prague (2010)

Great Britain-China Education Trust student award (2007)

PhD studentship. The University of Nottingham (2005)


在国际学术会议做重要报告等情况

Zhang X., Gonzalez-Carranza Z.H., Roberts J. A. (2007). Identifying potential target proteins for degradation regulated by the Hawaiian Skirt gene (HWS). 18th International Conference On Arabidopsis Research, Beijing, China, 2007. TAIR Publication: 501721786 (第18届国际拟南芥大会)

González-Carranza, Z.H., Zhang X., Roberts J.A. (2008). The F-box protein HAWAIIAN SKIRT regulates boundary fusion via miRNA processing. Comparative Biochemistry and Physiology, PART A, 150, P3.12 S189.

González-Carranza, Z.H., Zhang X., Peters J.L., Roberts J.A. (2009). Interaction between the ubiquitination and miRNA pathways in the regulation of flower development. 20th International Conference in Arabidopsis Research, P037, P12. (第20届国际拟南芥大会)

González-Carranza, Z.H., Zhang X., Peters J.L., Roberts J.A. (2009). ... Love me, love me not... a way to increase petal numbers from Arabidopsis. Workshop in mechanisms Controlling Flower Development, 31.

Zhang X., Marcos Lousa C.D., Baldwin S.A., Baker A., Theodoulou F.L. (2010). Crosskingdom Characterisation of Peroxisomal ABC transporters. Society for Experimental Biology annual meeting, Prague, CZ. (英国实验生物学学会,2010年会)

Zhang X., Marcos Lousa C.D., Baldwin S.A., Baker A., Theodoulou F.L. (2010). Crosskingdom Characterisation of Peroxisomal ABC transporters. Open European Peroxisome Meeting (OEPM 2010), Lunteren, The Netherlands. (欧洲过氧化物酶体会议)

Attendance to: GARNet 2010 meeting, Durham University, UK, September 2010 (英国实验生物学学会)BBSRC Next Generation Conference (Career Snapshots workshop), Manchester, UK, November 2010 (英国生物技术学会,青年学者会议)

Zhang X., Liu C. (2015) Post-translational modification of Arabidopsis S-adenosylmethionine synthetase (SAMS). Plant Metabolic Engineering-Harnessing Plant Metabolism for the Bio-Based Economy, Gordon Research Conference, July 19-24, 2015. Waterville Valley, Waterville Valley, NH

Zhang X., Liu C. (2017) The F-Box Protein-mediated Proteolytic Regulation of Flavonoid Biosynthesis in Arabidopsis. The 4th International Conference on Plant Metabolism (ICPM 2017) July 16-20, 2017. Dalian International Conference Center, China


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