教师名录

主要研究方向：

棉花抗病基因的筛选及抗病机制解析。

棉花黄萎病是一种土传真菌维管束病害，严重影响棉纤维的产量的品质。棉花抗病遗传资源的缺乏和黄萎病菌生理小种的多样性和变异性都严重制约了棉花抗病育种的研究和发展。本研究通过对已释放的棉花抗病相关转录组、蛋白组及代谢组数据进行重分析和交叉比对，从中挑选可能参与棉花抗病反应的候选基因，并利用病毒介导的基因沉默（VIGS）技术对候选基因进行抗病性鉴定。通过筛选，一共得到了24个调控棉花对黄萎病、枯萎病和灰霉病抗性的候选基因。这些基因分别参与棉花次生代谢、激素响应、活性氧ROS调节和细胞程序性死亡PCD等各个方面。本课题组利用CRISPR等技术在棉花中突变和超量表达了这些候选基因，并进行了进一步的功能解析和抗病分子机制研究，以期望通过此项研究，为棉花抗病育种提供理论基础和候选基因，并创制新的棉花抗病种质资源。

承担的主要研究项目：

1.主持国家自然科学基金青年项目：GbMPK3介导的棉花抗黄萎病机制研究(31701473) 2018-2020

2.参与（第二）国家自然科学基金青年项目：硝酸盐转运蛋白GbNRT2.1调节棉花抗旱的分子机理研究(31601344)在研2017-2019

3.参与（第二）国家自然科学基金面上项目：基于陆海渐渗系的棉花黄萎病成株抗性相关基因的鉴定与功能解析(31571724)在研2016-2019

4.主持河南省高等学校重点科研项目计划：GbMPK3基因调控棉花抗黄萎病的机制研究及在抗病育种中的应用(15A180028)已结项2015-2016

5.主持棉花生物学国家重点实验室开放课题：棉花GbMAPKKK1基因的克隆和功能分析(CB2015A31)已结项2015-2017

发表论文：

1.Lu Long^**, Dan-Dan Guo^**, Wei Gao, Wen-Wen Yang, Li-Pan Hou, Xiao-Nan Ma, Yu-Chen Miao, Jose Ramon Botella^*, Chun-Peng Song^*. Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression.Plant Methods2018,14:85 SCI（二区）

2.Lu Long^**,Jing-Ruo Zhao^**, Fu-Chun Xu, Wen-Wen Yang, Peng Liao, Ya Gao, Wei Gao^*, Chun-Peng Song. Silencing ofGbANSreduces cotton resistance toVertcillium dahliaethrough decreased ROS scavenging during the pathogen invasion process. Plant Cell, Tissue and Organ Culture (PCTOC) 2018,135(2):213-221. SCI（三区）

3.Fu-Chun Xu^**, Hui-li Liu^**, Yun-yun Xu, Jing-ruo Zhao, Ya-wei Guo,Lu Long, Wei Gao^*, Chun-peng Song. Heterogeneous expression of the cotton R2R3-MYB transcription factor GbMYB60 increases salt sensitivity in transgenicArabidopsis. Plant Cell, Tissue and Organ Culture (PCTOC) 2018, 133(1):15-25. SCI（三区）

4.Wei Gao^†, Fu-Chun Xu^†, Dan-Dan Guo, Jin-Ruo Zhao,Ji Liu,Ya-Wei Guo, Prashant K. Singh, Xiao-Nan Ma,Lu Long,Jose R. Botella, Chun-Peng Song^*. Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress.BMC plant biology2018, 18(1):15. SCI（二区）

5.Wei Gao^**,Lu Long^**, Xin-Quan Tian, Fu-Chun Xu, Ji Liu, Prashant K. Singh, Jose R. Botella, Chun-Peng Song^*. Genome Editing in Cotton with the CRISPR/Cas9 System.Front Plant Sci2017, 8:1364. SCI（二区）

6.Wei Gao,Lu Long, Xin-Quan Tian, Jing-Jing Jin, Hui-Li Liu, Hui Zhang, Fu-Chun Xu, Chun-Peng Song^*. Genome-wide identification and expression analysis of stress associated proteins (SAPs) containing A20/AN1 zinc-finger in cotton.Mol Genet Genomics2016, 291 (6):2199-2213. SCI（三区）

7.Wei Gao,Lu Long, Li Xu, Lindsey Keith, Xian-Long Zhang, Long-Fu Zhu^*. Suppression of the homeobox geneHDTF1enhances resistance toVerticillium dahliaeandBotrytis cinereain cotton.J Integr Plant Biol. 2016, 58(5):503-513. SCI（二区）

8.Wei Gao,Lu Long, Long-Fu Zhu^*, Li Xu, Wen-Hui Gao, Long-Qing Sun, Lin-Lin Liu, Xian-Long Zhang^*. Proteomic and virus-induced gene silencing (VIGS) analyses reveal that gossypol, brassinosteroids, and jasmonic acid contribute to the resistance of cotton toVerticillium dahliae. Molecular & cellular proteomics:MCP. 2013, 12 (12):3690-3703. SCI（一区TOP）

9.Lu Long, Wei Gao, Li Xu, Min Liu, Xiang-Ying Luo, Xin He, Xi-Yan Yang, Xian-Long Zhang, Long-Fu Zhu^*. GbMPK3, a mitogen-activated protein kinase from cotton, enhances drought and oxidative stress tolerance in tobacco. Plant Cell, Tissue and Organ Culture (PCTOC). 2014, 116 (2):153-162. SCI（三区）

10.Li Xu, Li Jin,Lu Long, Lin-Lin Liu, Xin He, Wei Gao, Long-Fu Zhu, Xian-Long Zhang^*. Overexpression of GbWRKY1 positively regulates the Pi starvation response by alteration of auxin sensitivity inArabidopsis.Plant Cell Reports, 2012, 31(12): 2177-2188.SCI（三区）

11.Li Xu, Long-Fu Zhu, Li-Li Tu, Lin-Lin Liu, Dao-Jun Yuan, Li Jin,Lu Long, Xian-Long Zhang^*. Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and histochemistry.Journal of Experimental Botany, 2011, 62(15): 5607-5621.SCI（二区）

12.Li Xu, Long-Fu Zhu, Li-Li Tu, Xiao-Ping Guo,Lu Long, Long-Qing Sun, Wei Gao, Xian-Long Zhang^*. Differential gene expression in cotton defence response toVerticillium dahliaeby SSH.Journal of Phytopathology, 2011, 159(9): 606-615.SCI（三区）

13.M. Farooq Hussain Munis, Li-Li Tu, Feng-Lin Deng, Jia-Fu Tan, Li Xu, Shi-Cheng Xu,Lu Long, Xian-Long Zhang. A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco. Biochem Biophys Res Commun. 2010, 393(1): 38-44.

14.高巍^#,刘会利^#,田新权,张慧,宋洁,杨勇,龙璐,宋纯鹏^*.海岛棉转录因子基因GbMYB60的克隆、表达及其抗逆功能研究.作物学报, 2016, 42(9): 1342-1351. 2016/6/27中文核心（A类）

15.杨勇^#,田新权^#,刘会利,宋洁,张慧,龙璐,高巍^*.海岛棉NAC转录因子基因GbNAC6的克隆和表达模式分析.棉花学报, 2017, 29(2):138-146. 2017/3/15 中文核心（C类）

16.张慧，田新权，高巍，蔡应繁，龙璐^*.陆地棉PPO的全基因组鉴定及对黄萎病菌的响应分析.棉花学报, 2017,29(5):428-436. 2017/9/15中文核心（C类）

专利：

1.朱龙付，高巍，龙璐，张献龙，袁道军，聂以春。棉花硬脂酰去饱和化酶GbSSI2基因及应用，CN201310019959.5。

2.朱龙付，张献龙，高巍，龙璐。棉花同源结构域转录因子基因GbHDTF1及应用，CN201310345168.1。

讲授课程：

《作物栽培与耕作》本科生课程

《植物生物技术实验》本科生课程

《生物化学实验》本科生课程

《棉花生物学专题》研究生课程

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