【研究方向】棉花抗病与发育机制及功能基因组学、遗传育种
【办公地点】棉花生物育种与综合利用全国重点实验室305室
【联系邮箱】cyf@henu.edu.cn;或yingfancai@outlook.com
【教育及工作经历】1986和1997年分别获西南大学(西南农业大学)学士和硕士学位,2003年获四川大学获博士学位。2005-2006年于美国康乃尔大学Boyce Thompson植物研究所和德州农工大学访问科学家(公派)。2013年8月至今,河南大学生命科学学院/棉花生物学国家重点实验室(棉花生物育种与综合利用全国重点实验室)“攀登计划”特聘教授。
【代表性论文】(#为共同一作,*为通讯作者):
Yi F, Song A, Cheng K, Liu J, Wang C, Shao L, Wu S, Wang P, Zhu J, Liang Z, Chang Y, Chu Z, Cai C, Zhang X, Wang P, Chen A, Xu J, Burritt DJ, Herrera-Estrella L, Tran LP*, Li W*,Cai Y*.Strigolactones positively regulate Verticillium wilt resistance in cotton via crosstalk with other hormones.Plant Physiol., 2023, 192: 945-966.
Cai Y, Cai X, Wang Q, Wang P, Zhang Y, Cai C, Xu Y, Wang K, Zhou Z, Wang C, Geng S, Li B, Dong Q, Hou Y, Wang H, Ai P, Liu Z, Yi F, Sun M, An G, Cheng J, Zhang Y, Shi Q, Xie Y, Shi X, Chang Y, Huang F, Chen Y, Hong S, Mi L, Sun Q, Zhang L, Zhou B, Peng R, Zhang X*, Liu F*. Genome sequencing of the Australian wild diploid speciesGossypium australehighlights disease resistance and delayed gland morphogenesis.Plant Biotechnology Journal. 2020,18:814-828.
Zhang L, Liu J, Cheng J, Sun Q#, Zhang Y, Liu J, Li H, Zhang Z, Wang P, Cai C, Chu Z, Zhang X, Yuan Y, Shi Y*,Cai Y*.lncRNA7andlncRNA2modulate cell wall defense genes to regulate cotton resistance to Verticillium wilt.Plant Physiol., 2022,189: 264-284.
Sun Q.; Xie Y.; Li H.; Liu J; Geng R.; Wang, P.; Chu Z.; Chang Y.; Li G.; Zhang X.; Yuan Y.;Cai Y. CottonGhBRC1regulates branching, flowering, and growth by integrating multiple hormone pathways,The Crop Journal, 2022,10: 75–87.
Ji G#, Liang C#,Cai Y#, Pan Z, Meng Z, Li Y, Jia Y, Miao Y, Pei X, Gong W, Wang X, Gao Q, Peng Z, Wang L, Sun J, Geng X, Wang P, Chen B, Wang P, Zhu T, He S*, Zhang R*, Du X*.A copy number variant at the HPDA-D12 locus confers compact plant architecture in cotton.New Phytol., 2021, 229:2091-2103.
Wang X#, Miao Y#,Cai Y#, Sun G, Jia Y, Song S, Pan Z, Zhang Y, Wang L, Fu G, Gao Q, Ji G, Wang P, Chen B, Peng Z, Zhang X, Wang X, Ding Y, Hu D, Geng X, Wang L, Pang B, Gong W, He S*, Du X*. Large-fragment insertion activates geneGaFZ(Ga08G0121), and is associated with the fuzz and trichome reduction in cotton (Gossypium arboreum).Plant Biotechnol J.2021, 19:1110-1124.
Peng S, Guo D, Guo Y, Zhao H, Mei J, Han Y, Guan R, Wang T, Song T, Sun K, Liu Y, Mao T, Chang H, Xue J,Cai Y, Chen D, Wang S*. CONSTITUTIVE EXPRESSER OF PATHOGENESIS-RELATED GENES 5 is an RNA-binding protein controlling plant immunity via an RNA processing complex.ThePlant Cell. 2022 Apr 26;34(5):1724-1744.
Cai C, Wang P, Zhao C, Lei W, Chu Z,Cai Y*, An G*.The use of ribosome-nascent chain complex-seq to reveal the translated mRNA profile and the role of ASN1 in resistance to Verticillium wilt in cotton.Genomics. 2021 Nov;113(6):3872-3880.
Ying Chang#, Bo Li#, Qian Shi, Rui Geng, Shuaipeng Geng, Jinlei Liu, Yuanyuan Zhang andYingfan Cai* Comprehensive Analysis of Respiratory Burst Oxidase Homologs (Rboh) Gene Family and Function of GbRboh5/18 on Verticillium Wilt Resistance inGossypium barbadense. September 2020,Frontiers in Genetics, 2020,11:788
Lin Zhang, Ye Tao, Suya Zhao, Xiaoyan Yin, Junmei Chen, Miao Wang,Yingfan Cai*,Qiuhong Niu*. A novel peroxiredoxin from the antagonistic endophytic bacterium Enterobacter sp. V1 contributes to cotton resistance againstVerticillium dahliae.Plant and Soil, volume 454, pages395-409 (2020)
Zhang L, Li W, Tao Y, Zhao S, Yao L,Cai Y*, Niu Q*.Overexpression of the Key Virulence Factor 1,3-1,4-β-d-Glucanase in the Endophytic Bacterium Bacillus halotolerans Y6 To Improve Verticillium Resistance in Cotton.Journal of Agricultural and Food Chemistry.
Dai P#, Miao Y#, He S, Pan Z, Jia Y, Cai Yingfan, Sun J, Wang L, Pang B, Wang M, Du Xiongming. Identifying favorable alleles for improving key agronomic traits in upland cotton.BMC Plant Biology, 2019 Apr 11;19(1):138.
Quan Sun, Guangfan Zhou,Yingfan Cai*, Yonghong Fan, Xiaoyan Zhu, Yihua Liu, Xiaohong He, Jinjuan Shen, Huaizhong Jiang, Daiwen Hu,Zheng Pan, Liuxin Xiang, Guanghua He,Jianping Yang. Transcriptome analysis of development of the stem in tumourous stem mustard,Brassica junceavar. tumida Tsen et Lee, by RNA sequencing.BMC Plant Biology, 2012, 12:53
Xiang Linxin, Liu J, Wu C, Deng Y, Cai C, Zhang X,Cai Yingfan*. Genome-wide comparative analysis of NBS-encoding genes in fourGossypiumspecies.BMC Genomics. 2017 Apr 12;18(1):292.
Quan Sun, Huaizhong Jiang, Xiaoyan Zhu, Xiaohong He, Liuxin Xiang, Yuzhen Shi, YouluYuan, Xiongming Du,Yingfan Cai*. Analysis of Sea-Island Cotton and Upland Cotton in Response toVerticillium dahliaeInfection by RNA Sequencing. 2013,BMC Genomics, 2013 ,14:852
Xu Zheng, Suowei Wu, Huqu Zhai, Peng Zhou, Meifang Song, Liang Su, Yulin Xi, Zhiyong Li,Yingfan Cai, Fanhua Meng, Li Yang, Haiyang Wang, Jianping Yang*. (2013). Arabidopsis Phytochrome B Promotes SPA1 Nuclear Accumulation to Repress Photomorphogenesis under Far-Red Light.The Plant Cell, 2013,25(1):115-33
Sun Q, Du X, Cai C, Long L, Zhang S, Qiao P, Wang W, Zhou K, Wang G, Liu X, Zhang H, Geng S, Yang C, Gao W, Mo J, Miao C, Song C*,Cai Yingfan*. To Be a Flower or Fruiting Branch: Insights Revealed by mRNA and Small RNA Transcriptomes from Different Cotton Developmental Stages.Scientific Reports. 2016 Mar 17;6:23212.
Yingfan Cai*, Yongfang Xie, Jinggao Liu. Glandless seed and glanded plant research in cotton.Agronomy for Sustainable Development,2010, 30(1): 181-190
Sun Quan, Wang G, Zhang X, Zhang X, Qiao P, Long L, Yuan Y*,Cai Yingfan*. Genome-wide identification of the TIFY gene family in three cultivated Gossypium species and the expression of JAZ genes.Scientific Reports. 2017 Feb 10;7:42418.
Ping Wang, Sai Zhang, Jing Qiao, Quan Sun, Qian Shi, Chaowei Cai, Jianchuan Mo, Zongyan Chu, Youlu Yuan, Xiongming Du, Yuchen Miao, Xiao Zhang and Yingfan Cai*. Functional analysis of theGbDWARF14gene associated with branching development in cotton. PeerJ,2019,DOI 10.7717/peerj.6901
Xiang Liuxin, Cai C, Cheng J, Wang L, Wu C, Shi Y, Luo J, He L, Deng Y, Zhang X, Yuan Y,Cai Yingfan* Identification of circularRNAs and their targets in Gossypium under Verticillium wilt stress based on RNA-seq.PeerJ. 2018 Mar 16;6:e4500.
Xiang Liuxin, Liu C, Luo J, He L, Deng Y, Yuan J, Wu C*,Cai Yingfan*. A tuber mustard AP2/ERF transcription factor gene, BjABR1, functioning in abscisic acid and abiotic stress responses, and evolutionary trajectory of the ABR1 homologous genes in Brassica species. PeerJ. 2018 Dec 11;6:e6071.
Sun Quan, Qiao J, Zhang S, He S, Shi Y, Yuan Y, Zhang X,Cai Yingfan*. Changes in DNA methylation assessed by genomic bisulfite sequencing suggest a role for DNA methylation in cotton fruiting branch development.PeerJ. 2018 Jun 14;6:e4945.
Wang W, Yuan Y, Yang C, Geng S, Sun Q, Long L, Cai C, Chu Z, Liu X, Wang G, Du X, Miao C, Zhang X,Cai Y*. Characterisation, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton.G3: Genes, Genomes, Genetics,2016 Nov 1. pii: g3.116.034512.
Xiaohong He, Quan Sun, Huaizhong Jiang, Xiaoyan Zhu, Jianchuan Mo, Lu Long,Liuxin Xiang, Yongfang Xie, Yuzhen Shi, Youlu Yuan,Yingfan Cai*. Identification of novel microRNAs in the Verticillium wilt-resistant upland cotton variety KV-1 by high-throughput sequencing.Springerplus. 2014 Sep 27;3:564.
Quan Sun,Yingfan Cai*, Yongfang Xie,Jianchuan Mo, Youlu Yuan, Yuzhen Shi , Shengwei Li, Huaizhong Jiang, Zheng Pan, Yunling Gao, Min Chen, Xiaohong He. Gene Expression Profiling During Gland Morphorgenesis in a Mutant and a Glandless Upland Cotton.Molecular biology reports,2010,37(7):3319–3325
Liuxin Xiang, Yuxian Xia*,Yingfan Cai*, Jijun Liu, Xiaohong He, Quan Sun, Xiaoyan Wang, Yuyin Fu, Yonghong Fan, Daiwen Dong, Guanfan Zhou, Jinjuan Shen and Yihua Liu. Characterisation of the first tuber mustard calmodulin-like gene, BjAAR1, and its functions in responses to abiotic stress and abscisic acid in Arabidopsis.Journal of Plant Biology, 2013, 56:168-175.
Yong-Fang Xie, Biao Li, Bochu Wang*,Ying-Fan Cai*. Construction of cDNA Library of Cotton Mutant (Xiangmian 18) Library During Gland Forming Stage.Colloids and Surfaces B: Biointerfaces, 2007, 60(2):258–263
Jinggao Liu, Robert Stipanovic,Yingfan Cai, Lorraine Puckhaber, Alois Bell. Cloning and Heterologous Expression of a Cytochrome P450 Hydroxylase Involved in the Terpenoid Biosynthetic Pathway in Cotton. Proceedings-Beltwide Cotton Conferences,USA. 2007, Jan.
Yingfan Cai,Hong Zhang,Yu Zeng,Jianchuan Mo,Jinku Bao,Chen Miao,Jie Bai,Fang Yan and Fang Chen*. An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton.Journal of Biosciences,2004, 29(1):67-71
承担主要项目:
1.国家重点研发计划子课题,棉花抗病虫基因资源发掘和利用(2022YFD1200303),80.0万元,研究期限:2022.12-2027.11(主持)
2.国家自然科学基金NSFC联合基金项目:棉花分枝发育相关基因GbBRC1等的功能解析(U1704104),52.0万元,2018.01-2020.12(主持)
3.国家自然科学基金面上项目,基于陆海渐渗系的棉花黄萎病成株抗性相关基因的鉴定与功能解析(31571724),64.0万元,研究期限:2016.01-2019.12(主持)
4.国家自然科学基金面上项目,陆地棉抗源抗落叶型黄萎病及相关抗病性应答的分子机理(31071461),33.0万元,研究期限:2011-2013(主持)
5.国家自然科学基金面上项目,棉属植物腺体形成相关基因的筛选与功能研究(30771311),29.0万元,研究期限:2008-2010(主持)
6.国家重点研发计划子课题负责人,棉花主要经济作物分子设计育种,重要农艺性状多基因互作网络解析(2016YFD0101902),129.0万元,研究期限:2016.07-2020.12(主持)
7.国家重点研发计划,黄河流域高效轻简化棉花新品种培育-优质多抗棉花新品种培育(2018YFD0100304),2018.06-2020.12(参加)
8.国家自然科学基金主任基金项目,棉属植物腺体形成相关基因的分离克隆与功能鉴定(30440032),研究期限:2005.1-2005.12(主持)
9.国家农业部项目,研究期限:2015-2016(主持)
10.国家863计划子课题(2008AA1OZ121-2),研究期限:2008-2011(主持)
11.国家农业部“发展棉花生产专项基金”项目-川棉65(200012),研究期限:2000-2004, 25.0万元
12.国家科技攻关项目(96-002-02-10-02),研究期限:1996-2000
13.四川省科技攻关项目,研究期限:1996-2002
14.四川省杰出青年科学基金项目(462),研究期限:2001.01-2003.12
15.重庆市重点攻关项目(CSTC,2011AB1095),研究期限:2011-2013
16.河南省科技创新杰出人才计划项目,研究期限:2015.01-2017.12
17.河南省自然科学基金项目,LncRNAs调控棉花黄萎病抗性的分子机制,研究期限:2022-2023
参编著作:
张相琼,蔡应繁.《四川棉花品种选育与主要品种介绍》(执笔撰写主要理论部分)。成都:天地出版社,2002年1月出版
周选围,林娟,舒坤贤,蔡应繁等.《生物技术概论》,“十二五”普通高等教育本科国家级规划教材.北京:高等教育出版社,2010年3月出版
授权发明专利:
棉花GbSLR1基因在植物根和分枝发育中的应用,发明专利,2021年7月6日,发明人:蔡应繁,张赛,孙全,何琳,乔婧,龙璐,高巍,张骁.ZL 2017 1,1135400.3,专利权人:河南大学
棉花GhLecRK1基因在植物抗黄萎病中的应用.ZL2017 1 1136716.4,发明专利2021-01-09。发明人:蔡应繁,龙璐,高巍,何琳,张慧,孙全,张骁.专利权人:河南大学
棉花GbABR1基因在抗黄萎病中的应用(发明名称),2016109549738(专利申请号),20161103(申请日),发明人:蔡应繁、刘鑫、龙璐、孙全、王微娜、张骁.专利权人:河南大学
棉花GbDREB基因在抗黄萎病中的应用,2016109549723(专利申请号),20161103,发明人:蔡应繁、刘鑫、龙璐、孙全、王微娜、张骁.专利权人:河南大学
棉花细胞色素P450 CYP94C1基因在抗黄萎病中的应用,2016108583214(专利申请号),20160928,发明人:蔡应繁、周克学、龙璐、王微娜、孙全、高巍、张骁、宋纯鹏.专利权人:河南大学
海岛棉GbNAC1基因在抗黄萎病中的应用,2016106897908(专利申请号),20160819,发明人:蔡应繁、王微娜、杨璨、耿帅鹏、孙全、张骁、宋纯鹏.专利权人:河南大学
【教学及人才培养】
1.棉花生物学专题(硕士、博士研究生课程)
2.文献阅读(硕士、博士研究生课程)
3.植物育种学(本科生课程)
4.作物栽培与耕作学(本科生课程)
【主要荣誉与成果】
1.国家审定品种:棉花抗枯黄萎病,抗红蜘蛛兼抗棉铃虫优质高产新品种“川棉239”的选育(长江流域国家品种区域试验通过的第一个双抗棉花品种)。本人排名第一。
2.国家技术发明奖:棉花抗黄萎病多菌系抗源种质川737、川2802的培育,成果第2完成人(成果完成者证书第2776号);1998年获国家发明三等奖(排名第3)。
3.四川省科技进步一等奖:抗棉黄萎病多菌系抗源种质的选育,成果第2完成人(成果完成者证书第2776号);1995年获四川省科技进步一等奖(排名第3)。
4.国家审定品种:棉花抗枯黄萎病兼抗棉铃虫优质高产新品种川棉243,1996年四川省审定,2000年又通过国家审定,成功转让品种专利。本人排名第二(共同主持)。
5.四川省审定品种:棉花抗黄枯萎病、抗红铃虫兼抗红蜘蛛高产优质新品种“川棉65”的选育,2000年获得农业部“农发专项资金”资助,2002年通过四川省审定。本人排名第一。
6.四川省科技进步一等奖:棉花高抗枯萎病抗源52-128、57-681的持久抗性研究与应用。2006年获四川省科技进步一等奖,本人排名第十。
7.四川省科技进步三等奖:棉花多抗病育种技术及系列新品种的培育,2004年获四川省科技进步三等奖。本人排名第五。