副教授

【研究方向】

单分子生物学、蛋白质机器、生物大分子结构动力学

【办公地点】

作物国重316

【联系邮箱】

wuwenqiang@henu.edu.cn

【教育及工作经历】

2009-2017西北农林科技大学本博连读（创新实验班）

2017至今 河南大学生命科学学院/省部共建作物逆境适应与改良国家重点实验室

【代表性论文】

1. Wu, W.Q., Hou, X.M.*, Li, M., Dou, S.X. and Xi, X.G.* (2015) BLM unfolds G-quadruplexes in different structural environments through different mechanisms.Nucleic Acids Research, 43, 4614-4626.

2. Wu, W.Q.*, Zhu, X. and Song, C.P.* (2019) Single-molecule technique: a revolutionary approach to exploring fundamental questions in plant science.New Phytologist, 223, 508-510.

3. Wu, W.-Q.^#* (Lead contact and co-first authors), Zhang, X.^#, Bai, D., Shan, S.-W., and Guo, L.-J.* (2022) Mechanistic insights into poly(C)-binding protein hnRNP K resolving i-motif DNA secondary structures.Journal of Biological Chemistry,298,102670.

4. Wang, L.^#, Xu, Y.-P.^#, Bai, D., Shan, S.-W., Xie, J., Li, Y., andWu, W.-Q.*(2022) Insights into the structural dynamics and helicase-catalyzed unfolding of plant RNA G-quadruplexes.Journal of Biological Chemistry, 298, 102165.

5. Wu, W.Q.^#, Zhang, M.L.^#, and Song, C.P.* (2020) A comprehensive evaluation of a typical plant telomeric G-quadruplex (G4) DNA reveals the dynamics of G4 formation, rearrangement, and unfolding.Journal of Biological Chemistry, 295, 5461-5469.

6. Bai, D., Shan, S.-W., Zhang, X., Li, Y., Xie, J., andWu, W.-Q.*(2023) Comprehensive insights into the structures and dynamics of plant telomeric G-quadruplexes.International Journal of Biological Macromolecules, 231, 123281.

7. Xue, Z.Y.^#,Wu, W.Q.^#*(Lead contact and co-first authors), Zhao, X.C., Kumar, A., Ran, X., Zhang, X.H., Zhang, Y., and Guo, L.J.*(2020) Single-molecule probing the duplex and G4 unwinding patterns of a RecD family helicase.International Journal of Biological Macromolecules,164,902-910.

8. Zhang, B.^#,Wu, W.Q.^#(co-first authors), Liu, N.N., Duan, X.L., Li, M., Dou, S.X., Hou, X.M.* and Xi, X.G.* (2016) G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction.Nucleic Acids Research, 44, 8385-8394.

9. Zhou, Y.^#, Zhang, T.^#, Wang, X.^#,Wu, W. Q.^#(co-first authors), Xing, J., Li, Z., Qiao, X., Zhang, C., Wang, X., Wang, G., Li, W., Bai, S., Li, Z., Suo, Y., Wang, J., Niu, Y., Zhang, J., Lan, C., Hu, Z., Li, B., Zhang, X., Wang, W., Galbraith, D. W., Chen, Y., Guo, S.*, and Song, C. P.*(2023) A maize epimerase modulates cell wall synthesis and glycosylation during stomatal morphogenesis.Nature Communications, 14, 4384.

10. Guo, A.Y., Zhang, Y.M., Wang, L., Bai, D., Xu, Y.P., andWu, W.Q.* (2021) Single-Molecule Imaging in Living Plant Cells: A Methodological Review.International Journal of Molecular Sciences, 22, 5071.

11. Zhang, M.L., Xu, Y.P., Kumar, A., Zhang, Y. andWu, W.Q.* (2019) Studying the Potassium-Induced G-Quadruplex DNA Folding Process Using Microscale Thermophoresis.Biochemistry, 58, 3955-3959.

12. Wu, W.Q., Hou, X.M., Zhang , B, Fossé, P., René, B. and Mauffret, O., Li, M., Dou, S.X.* and Xi, X.G.* (2017)Single-molecule studies reveal reciprocating of WRN helicase core along ssDNA during DNA unwinding.Scientific Reports, 7, 43954.

13. Hou, X.M.^#,Wu, W.Q.^#(co-first authors), Duan, X.L., Liu, N.N., Li, H.H., Fu, J., Dou, S.X.*, Li, M. and Xi, X.G.* (2015) Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase.Biochemical Journal, 466, 189-199.

【项目/著作/专利】

1. 国家自然基金面上项目(2022-2025)：植物特异解旋酶在RNA G-四链体介导mRNA成熟和翻译中的功能及分子机制(32171272)，主持

2. 国家自然基金青年项目(2019-2021)：单分子水平研究G-四链体RNA的结构动力学及其与eIF4A的相互作用(31800644)，主持

3. 河南省高等学校重点科研项目(2019-2020)：用单分子方法研究拟南芥端粒G-四链体DNA结构动力学和解旋机制(19A180014)，主持

【教学及人才培养】

指导研究生多人获得河南省优秀硕士学位论文、河南省优秀毕业生、河南大学优秀硕士学位论文、河南大学优秀毕业生、河南大学优秀研究生、河南大学优秀研究生干部等荣誉。硕士毕业生多人进入北京师范大学、厦门大学、武汉大学等单位攻读博士学位。

【主要荣誉】

1. 中原青年拔尖人才

2. 河南省高层次人才

3. 河南省优秀硕士学位论文指导教师

4. 教育部自然科学奖二等奖(第八完成人)

5. 陕西高等学校科学技术研究优秀成果一等奖(第三完成人)

6. 河南大学青年英才

7. 河南大学师德先进个人

8. 河南大学科研优秀奖

9. 河南大学优秀研究生指导教师

10. 河南大学优秀硕士学位论文指导教师